scLTdb is a comprehensive single-cell lineage tracing database with multi-functional modules for the analysis of gene expression, clonal compositions, fate outcomes, lineage relationships and potential regulators of cell fate determination. scLTdb collected 109 datasets including three species, 13 tissue sources, 2.8 million cells and 36 scLT technologies.
scLTdb provides:
1. Search and query scLT datasets by species, tissue sources, barcode types, and technologies.
2. Re-analyze and viualize scLT datasets through three interactive modules, including single cell module, lineage tracing module, and integration module.
3. Download well-processed scLT datasets in h5ad and rds format.
4. Online tools to analyze single cell lineage tracing and cellualr barcoding data.
5. A step-by-step tutorial for users to use scLTdb. Users can access our tutorial by clicking the Tutorial button located in the navigation bar.
Please note that when you click on the 'Explore dataset' button on the Search page, it may take approximately 30 seconds to load the dataset. This delay is caused by the large number of cells in the dataset and the limitations of computational resources. In case you encounter any difficulty loading the dataset, kindly refresh the page.
In recent years, the next-generation lineage tracing has involved cellular barcoding that uses a large number of synthetic DNA sequences to uniquely label cells, providing quantitative insights into stem cell dynamics and cell fate outcomes. This strategy can be achieved using various barcode types, including integration of exogenous random DNA sequences into the cell genome, recombination of endogenous DNA units, or genome editing-mediated DNA insertions and deletions (INDEL). Cellular barcoding allows researchers to distinguish individual cells based on specific DNA barcode sequences at clonal resolution. Combining cellular barcoding with single-cell genomics, also known as single-cell lineage tracing (scLT), has generated rich datasets that resolve cell fate and transcriptional or epigenetic state of each cell. In addition, using mutations and DNA variations that have accumulated in humans as natural barcodes, scLT is used to reconstruct high-resolution lineage trees in human hematopoiesis and cancers, which were lacking in medical research. Given the significance of scLT in biology and medicine, the publicly available scLT data call for in-depth and integrated analysis to yield new insights on cell fate determination in development and disease.
For users who need to know more about single cell lineage tracing, we recommand this review paper: https://www.nature.com/articles/s41576-020-0223-2 (Wagner et al, Nature Review Genetics, 2020).
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn
Explanation of key columns in 'obs' (h5ad data download) and 'metadata' (R data download) is provided below:
celltype: state annotation of cells
time information: temporal annotation of cells
experimental condition: experimental condition annotation of cells
mouse ID/patient ID: The mouse or patient source of cells
barcodes: cell lineage barcodes
raw_barcodes: raw lineage barcode sequences for Cas9-edited data
For users who have difficulty to download datasets from Cowtransfer, we also provide a ZENODO repository for them to access our well-processed single-cell lineage tracing datasets (https://zenodo.org/records/12176634).
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn
Contact:
School of Life Sciences, Westlake University
Weike Pei (correspondence): peiweike@westlake.edu.cn
Junyao Jiang (maintainer): jiangjunyao@westlake.edu.cn
Xing Ye (maintainer): yexing@mail.ustc.edu.cn